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. 2020 Nov 27;6(48):eabc9294. doi: 10.1126/sciadv.abc9294

Fig. 2. PGX3 is a touch-responsive pectin-degrading enzyme that mediates seedling thigmomorphogenetic changes.

Fig. 2

(A) RT-qPCR analysis of gene expression in response to mechanical resistance. Mean ± SD; n = 3. (B and C) Elastic modulus map (B) and quantification (C) of epidermal cell walls at the middle region of hypocotyls. The elastic modulus on the A-walls and P-walls was obtained by AFM in the QNM mode. Mean ± SD; n ≥ 10. ns, not significant. (D and E) Images (D) and hypocotyl length (E) of 3-day-old etiolated seedlings in response to mechanical resistance. Mean ± SD; n ≥ 20. (F) Mechanical forces inhibit PG activity via PGX3. Mean ± SD; n = 3. (G) Time-course analysis of changes in hypocotyl length in response to mechanical stimulation. Mean ± SD; n ≥ 20. (H) Reversible manner of PGX3 gene expression after 1 hour of touch treatment. Mean ± SD; n = 3. UT, untouched. (I and J) PGX3 gene expression (I) and PG activity (J) after removal of the touch stimulus. Seedlings were grown in the S condition (I) or in the low S condition (J) for 3 days, and then the lid was removed. Mean ± SD; n = 3. Lowercase letters above the bars in panels G and J represent significantly different groups, P < 0.05, one-way ANOVA and Tukey test.