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. 2020 Nov 27;6(48):eabc9294. doi: 10.1126/sciadv.abc9294

Fig. 5. PGX3 functions genetically downstream of EIN3 to regulate the morphology and soil emergence of etiolated seedlings.

Fig. 5

(A) Time-course analysis of the in vivo PG activity of etiolated seedlings in response to ethylene treatment. Three-day-old etiolated seedlings were treated with 10-ppm ethylene for the indicated periods. Relative PG activity was normalized to the absorbance of the WT–0 hour sample. Mean ± SD; n = 3. (B) In vivo relative PG activity of 3-day-old etiolated seedlings. The relative PG activity of the samples was normalized to the absorbance of samples from WT seedlings. Mean ± SD; n = 3. (C and D) Images (C) and hypocotyl length (D) of 3-day-old etiolated seedlings. Mean ± SD; n ≥ 20. (E and F) Soil emergence rate (E) and phenotype (F) of seedlings with soil cover. Seedlings were grown in the dark for 7 days on 1/2 MS medium without (0 mm) or with 4 mm sand cover, and the emergence rate was counted (E). Mean ± SE; n = 3. Soil emergence phenotypes were imaged following additional 2 days of light irradiation (F). Photo credit: Q. Wu, Peking University. Lowercase letters above the bars in panels B, D and E represent significantly different groups, P < 0.05, one-way ANOVA and Tukey test.