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. Author manuscript; available in PMC: 2020 Nov 27.
Published in final edited form as: Dev Cell. 2018 Jul 19;46(3):257–270.e5. doi: 10.1016/j.devcel.2018.06.020

Figure 2. Physical Contact Is the Cue Sufficient for Oriented AB Cell Division in Both C. elegans and Mouse.

Figure 2.

(A) Schematic illustration of contact reconstitution assay. Yellow dots with P1 cell indicate a midbody remnant.

(B) C. elegans AB division axis after the reconstitution of contact cues. Centrosomes (green), myosin (green), histone H2B (magenta), polystyrene bead (magenta), and cleavage furrow position (arrowheads) are shown along with the distribution of cleavage furrow orientations (bottom panel). Nocodazole 20 μg/mL was used.

(C) Mouse AB division axis in vivo and AB/CD division axis after contact reconstitution. Zona-free are embryos without zona pellucida. Cleavage furrow position (arrowheads) are shown along with the distribution of cleavage furrow orientation (bottom panel). Angle distributions are shown relative to the contact plane.

Scale bars, 10 μm. See also Figure S1.