Table 1.
Data collection and refinement statistics.
| Protein | Nup84-Nup133521–1157 VHH-SAN8 | Nup84-Nup133521–1157 VHH-SAN8 TeW derivative | Nup84-Nup133521–1157 | Nup84-Nup133521–1157 VHH-SAN8/9 | Nup13355–481 VHH-SAN4 | Nup13355–481 VHH-SAN5 |
|---|---|---|---|---|---|---|
| PDB code | 6X02 | – | – | 6X03 | 6X05 | 6X04 |
| Organism | S. cerevisiae, V. pacos | |||||
| Data collection | ||||||
| Space group | P212121 | P212121 | P212121 | P212121 | P21212 | P212121 |
| Cell dimensions | ||||||
| a, b, c (Å) | 72.3, 287.7, 297.4 | 72.5, 283.6, 295.7 | 73.7, 298.3, 310.0 | 72.3, 295.2, 295.6 | 93.4, 154.7, 41.4 | 85.7, 204.7, 205.2 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 103.4–6.4 (6.6–6.4)a | 147.8–7.8 (8.0–7.8) | 147.8–8.3 (8.6–8.3) | 147.8–7.4 (7.6–7.4) | 59.6–2.1 (2.2–2.1) | 49.7–2.7 (2.8–2.7) |
| Rp.i.m. | 2.2 (81.3) | 4.2 (42.0) | 3.9 (39.4) | 2.7 (61.7) | 5.2 (48.0) | 3.5 (68.4) |
| I/σ | 55.3 (1.0) | 13.8 (2.1) | 27.0 (1.8) | 46.6 (1.1) | 19.3 (1.5) | 29.1 (1.5) |
| CC1/2 | 1.00 (0.58) | 1.00 (0.89) | 0.99 (0.79) | 0.97 (0.54) | 1.00 (0.51) | 1.00 (0.57) |
| Completeness (%) | 99.8 (100.0) | 99.9 (100.0) | 99.8 (99.5) | 99.9 (100.0) | 99.9 (99.8) | 99.6 (97.3) |
| Redundancy | 24.1 (25.5) | 12.3 (12.9) | 6.8 (7.2) | 12.0 (12.7) | 11.9 (10.3) | 8.6 (8.3) |
| Refinement | ||||||
| Resolution (Å) | 103.4–6.4 | 147.8–7.4 | 59.6–2.1 | 49.7–2.7 | ||
| No. of Reflections | 13,453 | 9,375 | 35,967 | 100,268 | ||
| Rwork/RFree | 31.4/32.4 | 33.5/35.3 | 18.3/22.3 | 23.6/25.9 | ||
| No. of atoms | ||||||
| Protein | 6655 | 7106 | 3890 | 20,184 | ||
| Water | 0 | 0 | 285 | 17 | ||
| B factors (Å2) | ||||||
| Protein | 574.6 | 668.3 | 43.0 | 88.5 | ||
| Water | — | — | 43.0 | 46.9 | ||
| r.m.s. deviations | ||||||
| Bond length (Å) | 0.003 | 0.003 | 0.007 | 0.003 | ||
| Bond angles (°) | 0.84 | 0.81 | 0.87 | 0.57 | ||
aValues in parenthesis are for highest-resolution shell (10% of the data). One crystal was used for each dataset.