Table 6.
Table showing the primers designed and used to detect specific targets in the SARS-CoV-2 genome.
| Set | Primers | Sequence (5′->3′) | Length | Region |
|---|---|---|---|---|
| 1 | SARS-CoV2-Ampliseq-P1-18-41-F | TCCCAGGTAACAAACCAACCAACT | 24 | Leader |
| SARS-CoV-2-RP1-3-TRB-27531-27512 | AAATGGTGAATTGCCCTCGT | 20 | Orf7a | |
| 2 | SARS-CoV-2-FP2-TRB-27401-27425 | TTATTCTTTTCTTGGCACTGATAAC | 25 | Orf7a |
| SARS-CoV-2-RP1-3-TRB-27531-27512 | AAATGGTGAATTGCCCTCGT | 20 | Orf7a | |
| 3 | SARS-CoV2-Ampliseq-P1-18-41-F | TCCCAGGTAACAAACCAACCAACT | 24 | Leader |
| SARS-CoV-2_RP4_206_187 | GACGAAACCGTAAGCAGCCT | 20 | 5′ UTR | |
| 4 | SARS-CoV-2_FP4_79-99 | AAAATCTGTGTGGCTGTCACT | 21 | 5′ UTR |
| SARS-CoV-2_RP4_206_187 | GACGAAACCGTAAGCAGCCT | 20 | 5′ UTR | |
| 5 | SARS-CoV-2-FP2-TRB-27401-27425 | TTATTCTTTTCTTGGCACTGATAAC | 25 | Orf7a |
| SARS-CoV-2-RP1-2-TRB-27511-27491 | ATGTTCCAGAAGAGCAAGGTT | 21 | Orf7a |