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. 2020 Nov 27;3:724. doi: 10.1038/s42003-020-01434-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a (P)RR is expressed in both the cytoplasm and the nucleus in human PDAC cell lines and (P)RR-expressing HPDE-1/E6E7 cells. Immunofluorescence (IF) with anti-ATP6AP2 antibody recognizing a.a. 146–350, which covers from the extracellular to the cytoplasmic domains (×500). ECD Extracellular domain; TM Transmembrane domain; CYT Cytoplasmic domain. b (P)RR is expressed in the cytoplasm, as determined by IF with anti-ATP6AP2 antibody recognizing a.a. 224–237, which is localized in the extracellular domain (×500). c CTF of (P)RR is dominantly expressed in the insoluble nuclear fraction in human PDAC cell lines. Detection of each domain of (P)RR was performed using anti-ATP6AP2 antibody recognizing a.a. 146–350. Consistent results are obtained in three independent experiments. W Whole-cell lysates; C Cytoplasmic fraction; S Soluble nuclear fraction; I Insoluble nuclear fraction.