. 2020 Nov 27;11(1):4. doi: 10.1007/s13205-020-02534-5

Table 2.

Shoot (SDM), root (RDM) and nodule (NDM) dry mass, number of nodules per plant (NN) nitrogen content in the shoots (NCS), nitrogen accumulated in the shoots (NAS) of cowpea cv. BRS Pujante inoculated individually with 12 Bradyrhizobium spp. or co-inoculated (dual inoculation) with B. pachyrhizi BR 3262 and 11 Bradyrhizobium spp. of two experiments under the gnotobiotic condition (45 days after the emergence) and in non-sterile soil (52 days after the emergence) as substrates

Inoculation treatment	SDM	RDM	NN	NDM	NCS	NAS	SDM	RDM	NN	NDM	NCS	NAS
	g plant⁻¹		nod plant⁻¹	mg plant⁻¹	mg N g⁻¹	mg N plant⁻¹	g plant⁻¹		nod plant⁻¹	mg plant⁻¹	mg N g⁻¹	mg N plant⁻¹
	Gnotobiotic experiment (sterile sandas substrate)						Non-sterileconditions experiment (soilas substrate)
ESA 124	2.06a	1.66a	212b	307a	45c	94c	2.00a	1.06b	134c	197c	40b	82b
ESA 162	2.30a	1.29a	189b	289a	52c	119b	1.25a	0.89b	142c	219c	55b	64b
ESA 168	2.04a	1.22a	164b	208a	49c	106b	1.98a	1.12b	200c	277b	71a	150a
ESA 173	1.94a	1.30a	118b	173a	41c	79c	1.47a	0.62b	46d	96d	35b	50b
ESA 192	1.43b	1.22a	164b	222a	48c	69c	1.55a	0.93b	195c	259b	78a	121b
ESA 369	1.12b	1.14b	205b	167a	45c	51c	1.32a	0.93b	149c	221c	61b	82b
ESA 376	1.19b	1.34a	197b	302a	48c	57c	2.26a	1.26a	255b	381a	70a	165a
ESA 380	1.12b	1.21a	137b	132a	49c	55c	1.79a	0.99b	235b	378a	46b	89b
ESA 386	1.13b	1.13b	109b	166a	47c	53d	1.61a	0.81b	189c	286b	47b	77b
ESA 387	1.51b	1.23a	173b	244a	52c	78c	1.43a	0.57b	162c	298b	57b	83b
BR 3267	1.94a	1.38a	166b	172a	62b	118b	1.59a	1.51a	165c	162c	42b	67b
BR 3262	1.80a	1.50a	169b	206a	61b	111b	1.99a	1.58a	146c	205c	54b	113b
ESA 124 + bR 3262	2.42a	1.72a	170b	202a	63b	153a	1.90a	1.52a	315b	296b	76a	145a
ESA 162 + bR 3262	2.39a	1.85a	195b	190a	64b	154a	1.81a	1.54a	308b	513a	74a	132a
ESA 168 + bR 3262	2.13a	1.90a	191b	176a	65b	133b	1.21a	1.51a	268b	234c	90a	109b
ESA 173 + bR 3262	2.23a	1.33a	170b	172a	63b	141b	2.02a	1.42a	271b	270b	73a	149a
ESA 192 + bR 3262	2.48a	1.40a	172b	191a	67a	165a	1.99a	1.46a	284b	318b	77a	155a
ESA 369 + bR 3262	2.28a	2.22a	306a	333a	61b	139b	2.69a	1.26a	275b	285b	68a	194a
ESA 376 + bR 3262	2.26a	1.10b	156b	200a	56c	127b	2.18a	1.38a	291b	307b	81a	176a
ESA 380 + bR 3262	2.47a	1.26a	192b	211a	61b	152a	2.04a	1.20a	373a	417a	86a	177a
ESA 386 + bR 3262	2.18a	1.70a	192b	219a	54c	121b	2.08a	1.18a	417a	406a	95a	194a
ESA 387 + bR 3262	2.43a	1.36a	156b	186a	72a	172a	2.68a	1.29a	322b	290b	90a	247a
BR 3267 + bR 3262	2.43a	2.19a	266b	338a	76a	185a	2.44a	1.17a	149c	252b	60b	145a
N-fertilization*	2.62a	1.30b	0c	0b	64b	166a	2.08a	0.83b	6d	3d	85a	179a
Negativecontrol	0.54c	1.09c	0c	0b	30d	34 e	1.44a	0.98b	34d	60d	28c	40c
CV (%)	13.7	16.5	18.2	13.4	12.2	14.7	13.9	12.9	16.4	10.4	13.4	18.2

Data are an average of four replications

Means followed by the same letter, in the same variable and in the same experiment, do not differ by the Scott–Knott mean range test (p < 0.05). CV coefficient of variation

^*80 mg of N-NH₄NO₃ week⁻¹, from the second to the fifth week; ** 100 mg of N-NH₄NO₃ week⁻¹, from the second to the fifth week