Table 3.

Growth pattern of Bradyrhizobium spp. in the double layer spot test with two strains

Strain	BR 3262 in the bottom layer*		BR 3262 overlayed**
	YMA	oYMA	YMA	oYMA
ESA 124	+	+	±	−
ESA 162	+	±	±	±
ESA 168	+	±	±	−
ESA 173	+	+	±	−
ESA 192	+	+	±	−
ESA 367	±	±	±	±
ESA 376	+	±	±	±
ESA 380	±	±	±	±
ESA 386	+	+	+	±
ESA 387	±	±	±	±
BR 3267	±	+	±	±
BR 3262	±	±

+ Synergism (positive growth of the second layer near the first layer colonies); ±  no influence of the strain in the bottom layer on the overlayed strain; − Antagonism (bacteria in the bottom layer inhibiting the growth of the overlayed strain). YMA = standard YMA medium; oYMA = 1/5 strength YMA medium

*BR 3262 in the spots of the bottom layer and the bacteria in the first collum overlayed (in this case, testing the antagonism/synergism of BR 3262 on the other strains);

**The strains in the first collum in the spots of the bottom layer and BR 3262 overlayed (in this case, testing the antagonism/synergism of the strains on BR 3262)