Table 2.
Effect of some metal ions, chelating agents, and some inhibitors/activators on the partially purified NYSO laccase
| Relative activity (%) (mean ± SD) | ||
|---|---|---|
| Tested | Metal ion concentration | |
| 1 mM | 10 mM | |
| Control | 100 ± 0 | 100 ± 0 |
| MgCl2 | 157.8 ± 0.76 | 127.8 ± 0.75 |
| CrCl2 | 169.2 ± 2.25 | 148.7 ± 1.52 |
| MnCl2 | 140.1 ± 0.76 | 151.9 ± 1.70 |
| HgCl2 | 60.1 ± 0.76 | 59.9 ± 0.66 |
| CuSO4.5H2O | 171.1 ± 0.89 | 241.8 ± 2.02 |
| FeSO4.7.H2O | 15.6 ± 0.58 | 10.9 ± 0.85 |
| NiSO4 | 141.2 ± 1.10 | 198.5 ± 1.25 |
| CdSO4 | 167.9 ± 1.76 | 166.5 ± 1.36 |
| ZnSO4.7H2O | 177.4 ± 2.16 | 171.8 ± 2.01 |
| CoSO4 | 150.6 ± 0.65 | 191.3 ± 1.42 |
| Chelating agent concentration | ||
| 1 mM | 10 mM | |
| EDTA | 215.6 ± 1.25 | 24.6 ± 0.53 |
| Sodium citrate | 114.7 ± 0.3 | 99.4 ± 0.51 |
| Sodium oxalate | 103.6 ± 2.36 | 95.9 ± 1.79 |
| Inhibitor/activator concentration | ||
| 1 mM | 10 mM | |
| Sodium azide | 69.0 ± 0.10 | 20.5 ± 0.50 |
| DTT | 7.8 ± 2.02 | 5.5 ± 0.47 |
| Urea | 157.3 ± 2.33 | 127. ±1.67 |
| Imidazole | 140.7 ± 1.04 | 125.4 ± 1.58 |
| Kojic acid | 125.3 ± 2.0 | 71.1 ± 1.27 |
| Cysteine-HCl | 9.3 ± 1.10 | 2.1 ± 0.1 |
| β-Mercaptoethanol | 7.4 ± 0.52 | 4.6 ± 0.5 |
| Thioglycolic acid | 11.4 ± 1.26 | 9.6 ± 0.5 |
| PMSF | 106.2 ± 1.04 | 30.1 ± 0.6 |
| H2O2 | 100.8 ± 2.25 | 89.5 ± 0.5 |
| Triton X-100 | 140.0 ± 0.95 | 116.2 ± 1.31 |
| SDS | 2.9 ± 0.1 | 0.60 ± 0.045 |