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. 2020 Nov 27;11:6080. doi: 10.1038/s41467-020-19486-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a Calculation of the theoretical plasma concentration. b Hemolytic activity of T-cell-targeted mRNA nanoparticles (NPs). c Quantitative determination of complement activation by an Enzyme Immunoassay. A 2-fold change relative to the negative PBS control was defined as the assay threshold (dashed line). d Mitochondrial oxidative stress in lymphocytes following NP transfection. In all panels of this figure, N = 9 biologically independent blood samples were analyzed. Also shown are the mean values ± SD. Pairwise differences between groups were analyzed by two tailed unpaired Student’s t-test.