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. 2020 Nov 27;3:717. doi: 10.1038/s42003-020-01449-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Immunofluorescence images of MSCs cells cultured for 3 h onto functionalized substrates (FN-coated), serum-depleted and borax (PLLA-B2%, PLLA-B5%) in culture medium. Images show actin cytoskeleton (green) and α₅ or α_v integrins (magenta). Borax induces the expression of FN-binding integrins. Scale bar 25 µm. Right graphs: image analysis quantification of α₅ and α_v integrins levels. Twenty images/condition from three different biological replicas were analyzed. The data represented in graphs correspond to n ≥ 7. b qPCR analysis of relative mRNA expression of FN-binding integrins (α₅β₁ and α_vβ₃) before and after silencing of NaBC1 transporter. Active NaBC1 induces FN-binding integrins expression. n ≥ 3 different biological replicas. Statistics are shown as mean ± standard deviation. Data were analyzed by an ordinary one-way ANOVA test and corrected for multiple comparisons using Tukey analysis (P = 0.05). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.