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. 2020 Nov 27;3:717. doi: 10.1038/s42003-020-01449-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Images of nuclei (cyan) and co-localization dots (magenta) of MSCs cultured for 3 h onto functionalized substrates (FN-coated), serum-depleted and borax (PLLA-B2%, PLLA-B5%) in the culture medium. Scale bar represents 10 µm. b Image analysis quantification of the number of co-localization dots of NaBC1/α₅, NaBC1/α_v and NaBC1/BMPR1A. Co-localization levels increased with borax concentration. Thirty images/condition from three different biological replicas were analyzed. The data represented in graphs correspond to n ≥ 18. Statistics are shown as mean ± standard deviation. Data were analyzed by an ordinary one-way ANOVA test and corrected for multiple comparisons using Tukey analysis (P = 0.05). **p < 0.01, ***p < 0.001, ****p < 0.0001.