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. 2020 Nov 27;3:717. doi: 10.1038/s42003-020-01449-4

Fig. 6. Effects of active-NaBC1 in nuclear morphology.

Fig. 6

Immunofluorescence images of MSCs cells cultured for 3 h onto functionalized substrates (FN-coated), serum-depleted and borax (PLLA-B2%, PLLA-B5%) in culture medium. Images show actin cytoskeleton (gray) and nuclei (cyan). Borax induces nuclear stress resulting in decreased nuclear circularity, higher nuclear size and aspect ratio (AR), and increased actin stress fibers over the nucleus. Scale bar 20 µm. Image analysis quantification of nuclear circularity, nuclear size, nuclear aspect ratio (AR) and nuclear actin stress fibers. Twenty images/condition from three different biological replicas were analyzed. The data represented in graphs correspond to n ≥ 10. Statistics are shown as mean ± standard deviation. Data were analyzed by an ordinary one-way ANOVA test and corrected for multiple comparisons using Tukey analysis (P = 0.05). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.