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. 2020 Nov 27;3:717. doi: 10.1038/s42003-020-01449-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Immunofluorescence images of MSCs cultured for 15 days onto functionalized substrates (FN-coated) and borax (PLLA-B2%, PLLA-B5%) in culture medium, under basal and differentiation conditions (osteogenic), for late osteogenic detection markers (OPN and ALP-magenta, nuclei-cyan). Scale bar 25 µm. Oil red O staining of MSCs cultured for 15 days onto functionalized substrates (FN-coated) and borax (PLLA-B2%, PLLA-B5%) in culture medium, under basal and differentiation conditions (adipogenic), for adipocyte detection showing typical rounded adipocyte shape and accumulation of intracellular fat droplets. Scale bar 25 µm. b qPCR analysis of relative mRNA expression of late expressed markers involved in osteogenic (OPN) and adipogenic (LPL) lineage determination. RNA was extracted after 15 days of culture under both basal and differentiation conditions (osteogenic and adipogenic differentiation media). Statistics are shown as mean ± standard deviation. n ≥ 3 different biological replicas. Data were analyzed by an ordinary one-way ANOVA test and corrected for multiple comparisons using Tukey analysis (P = 0.05). *p < 0.05, **p < 0.01, ***p < 0.001.