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. 2020 Nov 27;11:6045. doi: 10.1038/s41467-020-19874-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Illustration of the strategy to express hM3Dq or hM4Di in TEE-tagged dDG or vDG neurons for chemogenetic manipulation. b The timeline of the chemogenetic activation experiment. CNO, clozapine-N-oxide. n = 15 mice per group. c Representative images from the dDG-CNO group (upper panel) and the vDG-CNO group (lower panel) show c-fos and mCherry immunostaining in dDG and vDG respectively. Arrows indicate activated granule cells that were labeled during tactile enrichment. Scale bar = 20 μm. d, e At 4 weeks after tactile enrichment, chemogenetic activation of TEE-tagged dDG but not vDG neurons enhanced performance in the object location task (d), whereas activation of TEE-tagged vDG but not dDG neurons increased the time in open arms and the number of head dips in the elevated plus maze (e). f The timeline of the chemogenetic inhibition experiment. n = 10–11 mice per group. g Representative images show the absence of colocalization between c-fos and mCherry in dDG or vDG after CNO administration. Scale bar = 20 μm. h, i At 1 week after tactile enrichment, mice with inactivation of TEE-tagged dDG but not vDG neurons failed to discriminate the objects, indicative of impaired spatial memory (h). Inactivation of TEE-tagged vDG but not dDG neurons reduced the time in open arms and the number of head dips in the elevated plus maze (i). *P < 0.05, **P < 0.01, ***P < 0.001. ^&&P < 0.01 for pairwise comparisons between the percentage of time exploring the relocated and the stationary objects. Data are presented as mean ± SEM. Detailed statistics are provided in Supplementary Table 2. Source data are provided as a Source Data file.