Table 2.

Relative protein expression in the heart tissue of 26-week-old low-density lipoprotein receptor knockout (Ldlr^-/-) mice.

Parameter	P0.3D50	P0.3D1000	P1.5D50	P1.5D1000	2-WA p-values
					P	D	P × D
Relative protein expression (reference protein: GAPDH)
IR	0.92  ±  0.17	1.00  ± 0.24	1.06 ± 0.22	1.11 ± 0.27	0.216	0.410	0.990
IGF1R	0.99 ± 0.25	1.01 ± 0.24	1.01 ± 0.39	1.08 ± 0.33	0.975	0.465	0.687
AKT1	1.00 ± 0.13	0.95 ± 0.20	1.05 ± 0.12	1.06 ± 0.15	0.322	0.625	0.596

Mice were fed a Western diet with adequate (0.3%; P_0.3) or high (1.5%; P_1.5) phosphorus levels coupled with adequate (1000 IU; D₁₀₀₀) or low (50 IU; D₅₀) vitamin D levels over a 17-week period. The expression of the insulin receptor (IR), the insulin-like growth factor 1 receptor (IGF1R) and the RAC-alpha serine/threonine-protein kinase (AKT1) are shown.

Data are provided as mean ± standard deviation [n = 14]. Data were analysed via two-factorial analysis of variance (2-WA) (significance level p < 0.05) with the classification factors phosphorus and vitamin D as well as their interaction.