Fig. 1. Specific H. pylori strains form biofilms in rich media.

Biofilm formation of H. pylori strains was assessed using the microtiter plate crystal violet biofilm assay. Strains were grown for three days in BB10, with no shaking, under 10% CO₂, 5% O₂ and 85% N₂. Results represent the crystal violet absorbance at 595 nm, which reflects the biofilm biomass. Experiments were performed three independent times with at least six technical replicates for each. Error bars represent standard error of the mean.