Fig. 8. Biofilm formation by H. pylori G27 WT and its isogenic mutants on AGS cells surface.

Biofilm formation was performed in 24-well plates with 85% AGS cells confluency. Bacterial numbers were determined by plating (CFU/ml) after 1 h (A) and three days (B) of coincubation. Experiments were performed three independent times with triplicates each time. Error bars represent standard deviations for each average value. Statistical analyses were performed using one-way ANOVA with Tukey post hoc test (*P < 0.05, ****P < 0.0001).