Table 4.
Strains used for this in the present study.
| Strain | KO reference no. | Genotype or description | Reference and/or source(s) |
|---|---|---|---|
| G27 | KO379 | WT strain | N. Salama (Fred Hutchison Cancer Research Center, USA) |
| SS1 | KO457 | WT strain | J. O’Rourke (University of New South Wales, Australia) |
| J99 | KO479 | WT strain | N. Salama (Fred Hutchison Cancer Research Center, USA) |
| 26695 | KO668 | WT strain | ATCC 700392 |
| X472AL | KO612 | WT strain | D. Berg (Washington University, USA) |
| M6 | KO613 | WT strain | E. Joyce, A. Wright (Tufts University School of Medicine, USA) |
| CYP34 (CYP3401) | KO637 | WT strain | D. Berg |
| HP43504 | KO669 | WT/clinical isolate | NTCT 11637 |
| HP 100-1 | KO763 | WT/clinical isolate | J. Solnick (UC Davis, USA) |
| HP 12-1 | KO760 | WT/clinical isolate | J. Solnick |
| HP 28-1 | KO761 | WT/clinical isolate | J. Solnick |
| HP116-1 | KO764 | WT/clinical isolate | J. Solnick |
| HP 125-2 | KO765 | WT/clinical isolate | J. Solnick |
| HP 208-2 | KO767 | WT/clinical isolate | J. Solnick |
| G27 ΔHPG27_166a | KO1635 | ΔHPG27_166:aphA3 | This study |
| G27 ΔHPG27_526a | KO1637 | ΔHPG27_526::cat | This study |
| G27 ΔhydEa | KO1638 | ΔhydE::cat | This study |
| G27 ΔHPG27_1233a | KO1639 | ΔHPG27_1233::cat | This study |
| G27 ΔHPG27_1066a | KO1640 | ΔHPG27_1066::cat | This study |
| G27 ΔacsAa,b | KO1641 | ΔacsA::cat | This study |
| G27 ΔacxABa | KO1642 | ΔacxAB::cat | This study |
| G27 ΔHPG27_715a | KO1644 | ΔHPG27_715::cat | This study |
| G27 ΔmotB | KO489 | ΔmotB::kan-sac2 | This study |
| G27 ΔfliA | KO1285 | ΔfliA::kan | K. Guillemin (University of Oregon, USA) |
WT wild-type, aphA3 kanamycin resistant, cat chloramphenicol resistant, ATCC American Type Culture Collection.
aAll transformations were performed using natural transformation using plasmid constructs (pTwist_Amp) in which the gene’s coding sequence was completely or partially removed and replaced by a kanamycin or chloramphenicol-resistance cassette using allelic exchange.
bMutant ΔacsA showed a slight defect in growth.