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. 2020 Nov 27;11(11):1016. doi: 10.1038/s41419-020-03222-1

Fig. 7. HMPL-013 inhibits HCVECs derived CCL2-induced macrophage migration and M1 polarization.

Fig. 7

HCVECs were randomly assigned into six groups: normal, hypoxia (24 h), hypoxia + DMSO, hypoxia + CCL2-neutralizing antibody (5 μg/ml for 30 min), hypoxia + HMPL-013, and hypoxia + HMPL-013 + recombinant mouse CCL2 protein (100 ng/ml for 24 h). a ELISA was done to detect CCL2 protein level in the macrophage culture supernatant. ***P < 0.005 vs. normal group; ##P < 0.01 and $$P < 0.01 vs. hypoxia group; %%P < 0.01 vs. hypoxia + HMPL-013 group. b Western blot was done to detect CCR2 protein level in the macrophage lysates. c Densitometry values of CCR2 normalized to GAPDH. ***P < 0.005 vs. normal group; ###P < 0.005 and $$P < 0.01 vs. hypoxia group; %P < 0.05 vs. hypoxia + HMPL-013 group. d Transwell assay was done to detect macrophage migration. e The average number of migrated macrophages was analyzed. f Western blot was done to detect NOS2 and Arg1 protein level in the macrophage lysates. g Densitometry values of each molecule normalized to GAPDH. ***P < 0.005 vs. normal group; ##P < 0.01, $$$P < 0.005, and $$P < 0.01 vs. hypoxia group; %%P < 0.01 vs. hypoxia + HMPL-013 group. n = 4 in each group.