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. 2020 Nov 23;23(12):101786. doi: 10.1016/j.isci.2020.101786

Dynamic 13C Flux Analysis Captures the Reorganization of Adipocyte Glucose Metabolism in Response to Insulin

Lake-Ee Quek , James R Krycer, Satoshi Ohno, Katsuyuki Yugi, Daniel J Fazakerley, Richard Scalzo, Sarah D Elkington, Ziwei Dai, Akiyoshi Hirayama, Satsuki Ikeda, Futaba Shoji, Kumi Suzuki, Jason W Locasale, Tomoyoshi Soga, David E James ∗∗, Shinya Kuroda ∗∗∗
PMCID: PMC7695902  PMID: 33294794

Main Text

(iScience 23, 100855-1–100855-19; February 21, 2020)

In Figure 7B of the originally published version of this article, the authors sourced some data from a previously published paper (Krycer et al., 2020). However, they inadvertently failed to acknowledge this and cite the source. This has since been corrected online, and the source is now cited in the legend of Figure 7B. The reference has also been added. The authors sincerely apologize for this oversight.

Figure 7. Insulin Altered the Profile of Substrates’ Fates

(B) The partitioning of radiolabelled glucose into end products CO2, glycerol (TAG-Gly) and fatty acyl. These pools constituted a small fraction of the glucose consumed compared to lactate efflux determined by enzymatic assay. The rates for glucose, lactate, and CO2 were sourced from Krycer et al. (2020), showing glucose uptake, lactate production, and glucose oxidation into CO2 rates from adipocytes cultured in 10 mM glucose with and without 100 nM insulin.

Contributor Information

Lake-Ee Quek, Email: lake-ee.quek@sydney.edu.au.

David E. James, Email: david.james@sydney.edu.au.

Shinya Kuroda, Email: skuroda@bs.s.u-tokyo.ac.jp.

References

  1. Krycer J.R., Quek L.-E., Francis D., Fazakerley D.J., Elkington S.D., Diaz-Vegas A., Cooke K.C., Weiss F.C., Duan X., Kurdyukov S. Lactate production is a prioritized feature of adipocyte metabolism. J. Biol. Chem. 2020;295:83–89. doi: 10.1074/jbc.RA119.011178. [DOI] [PMC free article] [PubMed] [Google Scholar]

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