Table 1.

Summary of the gene expression control systems in CFPS.

Sorting	Physical signal	Key elements	Mechanism	Induction Multiple	Ref.
Direct response	Light	Two-component system: YF1/FixJ		6	[5]
		One-component system: EL222		>10	[17]
		Azobenzene-tethered photoresponsive T7 promoter		7.1	[30]
	Temperature	RNA thermometer		11	[18]
Indirect response	Electric field	DNA compartment on a chip		–	[19]
		DMF board		–	[42]
	Magnetic force	Magnetic beads coated with DNA		–	[20]