Figure 4.

MT1-MMP Y573D Mice Show Decreased Cortical Bone and Increased Trabecular Bone

(A and B) MicroCT analysis of cortical (A) and trabecular bone (B) from Mmp14^wt/wt (wt/wt; n = 3) and Mmp14^Y573D/Y573D (YD/YD; n = 3) 3-month-old, male mice. Upper panels; 3D reconstruction; lower panels: quantitative analysis. The histograms show mean ± S.D. (A) BV/TV: bone volume/tissue volume; ∗: p = 0.0067; Cs. Th.: cortical thickness; ∗∗p = 0.0009; M. Ar.: bone marrow area; ∗∗∗p = 0.0524. (B) BV: bone volume; †: p = 0.0115; BV/TV: bone volume/tissue volume; #: p = 0.0366; Tb. Th.: trabecular thickness; Ӿ: p = 0.0290. All p values by two-tailed Student's t-test.

(C) Representative sections of the femurs of Mmp14^wt/wt (wt/wt), Mmp14^Y573D/wt (wt/YD) and Mmp14^Y573D/Y573D (YD/YD) 2-month-old, male mice. H&E staining; scale bar, 1 mm.

(D) Osteomeasure analysis of trabecular bone in femurs (blue bars) and tibiae (red bars) of the mice shown in panel (C) Mean ± s.d. of 5 mice/group is shown. BV/TV: bone volume/total volume; ∗: p = 0.0329; ∗∗: p = 0.0012; #: p = 0.0489. Tb. N.: number of trabecule; ∗: p = 0.0425; ∗∗: p = 0.0021; #: p = 0.0018. Tb. Th.: trabecular thickness; ∗: p = 0.0478; ∗∗: p = 0.0018; #: p = 0.0089. Tb. Sp.: space between trabecule; ∗: p = 0.0014; ∗∗: p = 0.0019; #: p = 0.0197 (sample vs corresponding wt control). All p values by two-tailed Student's t-test.

(E) RNA-seq analysis of differential gene expression in bone from Mmp14^Y573D/Y573Dvs. Mmp14^wt/wt mice. Volcano plot representing genes with a significant (p ≤ 0.05) fold change higher than 2 (red dots) or lower than - 2 (green dots).

(F) GO analysis of pathways overrepresented in the bone of Mmp14^Y573D/Y573Dvs. Mmp14^wt/wt 3-months-old male mice.

See also Figure S2.