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. 2020 Nov 11;21(22):8494. doi: 10.3390/ijms21228494

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sperm accumulation in oocytes’ perivitelline space (PVS) after control oocytes (Zp3-Cre ^−/− Itgb1 ^flox/flox) insemination with sperm from KD (Sycp1-Cre ^+/− Itgb1 ^flox/flox) or control (Sycp1-Cre ^−/− Itgb1 ^flox/flox) males. After cumulus-intact IVF assays, was reported the percentage of PVS containing-oocytes (mean ± s.e.m.) that showed high and significant difference when comparing the two groups (*** p < 0.0001). The numbers in parentheses represent the number of used oocytes in each group.