Table 3.

Investigating role of NaBH₃CN concentration and reaction time on GACox conjugation to CRM₁₉₇.

[NaBH3CN] in Reaction (mg/mL)	Reaction Time	GAC/CRM197 w/w Ratio in Purified Conjugate
10	4 h	0.34
	ON	0.41
	2 days	0.39
5	4 h	0.36
	ON	0.46
	2 days	0.48
1	4 h	0.42
	ON	0.45
	2 days	0.47