Figure 6.

Cancer-associated expression of Pcmt1/PIMT: (a) Output from GEPIA analysis of Pcmt1 expression in healthy versus tumor tissues (http://gepia.cancer-pku.cn/about.html) [40]. GEPIA is a web server for analyzing the RNA sequencing expression data of 9736 tumors and 8587 normal samples from the TCGA and the GTEx projects, using a standard processing pipeline. Increased tumor-associated Pcmt1 levels were shown in a wide spectrum of cancer-types: BRCA-breast invasive carcinoma, CESC-cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD-colon adenocarcinoma, ESCA-esophageal carcinoma, HNSC-head and neck squamous adenocarcinoma, LIHC-liver hepatocellular carcinoma, LUSC-lung squamous cell carcinoma, PAAD-pancreatic adenocarcinoma, PRAD-prostate adenocarcinoma, READ-rectum adenocarcinoma, STAD-stomach adenocarcinoma, UCS-uterine carcinosarcoma. Data were expressed in Log2 scale, n = number of samples. (b) Output from GENEVESTIGATOR analysis of Pcmt1 and CA9 gene expression levels in various cancer types (https://genevestigator.com/). Data were selected from HS_AFFY_U133PLUS_2-0, analyzed using Cancer/ScatterplotTree settings and expressed as Log2. (c) Representative image of immunohistochemical staining of CAIX and PIMT in CRC patient tissue sample. Images were taken using objective 40×. (d) Representative image showing the interaction between PIMT and CAIX protein in CRC patient tissue samples detected by proximity ligation assay. Red PLA signals indicating the interaction of CAIX with PIMT, nuclei are blue. Negative control was prepared by omission of the PIMT primary antibody. Images were taken using objective 40× and zoom 3.