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. 2020 Nov 16;12(11):3527. doi: 10.3390/nu12113527

Table 4.

Protein requirements of female athletes completing intermittent exercise.

Author/Year (Study Design) Female Athletes Menstrual Cycle/Contraceptives Exercise Protocol Protein Dose, Type, Timing Control/Comparison Intake Outcome(s) from Protein Intake 1
Daily Protein Requirements
Wooding et al. 2017
[48] (cross-over)
Six competitive rowing, ice hockey, volleyball athletes
68.8 ± 4.1 kg
Body fat 21.8 ± 2.7%
Luteal (days NR) Modified Loughborough test (4 × 15 min variable intensity shuttle run) Isocaloric meal with 0.2–2.66 g/kg/day crystalline amino acids based on egg protein provided in eight hourly doses post-exercise
(% energy NR)
N/A EAR 1.41 g/kg/day
RDI 2 1.75 g/kg/day
Acute Protein Requirements
Brown et al. 2018 [37]
(cohort)
20 competitive dancers (n = 10 intervention, n = 10 control)
61.8 ± 7.9 kg
Body fat % NR
Six luteal,
14 hormonal contraceptives (groups NR)
15 × 30 m repeated sprints 0.32 g/kg whey protein immediately and 2 h post-exercise (energy 91% protein, 8% CHO, 1% fat)
(average daily protein intake of 1.8 g/kg/day. Daily energy: 21% protein, 63% CHO, 24% fat)
0.32 g/kg carbohydrate immediately and 2 h post-exercise (energy 0% protein, 99.5% CHO, 0.5% fat)
(daily protein intake 1.3 g/kg/day. Daily energy: 15% protein, 61% CHO, 25% fat)
↓ decline in reactive strength index during 72 h post-exercise
↓ CK levels at 24 h post-exercise
Wilborn et al. 2016 [47] (single intervention) 3 Nine resistance-trained athletes
65.1 ± 8.4 kg
Body fat 25.5 ± 7.2%
NR Eight-week whole-body intermittent exercise program,
four sessions/week
0.38 g/kg whey protein post-exercise (energy 96% protein, 4% CHO, 0% fat)
(average daily protein intake 1.1 g/kg/day)
N/A ↑ maximal strength and agility
Taylor et al. 2016 [44]
(cohort)
14 competitive basketballers (n = 8 intervention
66.0 ± 3.1 kg, body fat 25.4 ± 4.2%; n = 6 control 68.2 ± 7.6 kg, body fat 25.1 ± 4.7%)
NR Eight-week whole body anaerobic, agility and RT program,
four sessions/week
0.36 g/kg whey protein pre- and post-exercise (energy 96% protein, 0% CHO, 4% fat) (average daily protein intake 1.39 g/kg/day) 0.35 g/kg maltodextrin pre- and post-exercise (energy 0% protein, 100% CHO, 0% fat) (daily protein intake of 1.08 g/kg/day) ↑ maximal strength and agility scores vs. control
Wilborn et al. 2013 [46]
(cohort)
16 competitive basketballers (n = 8 intervention 66.0 ± 4.9 kg, body fat 27.0 ± 4.9%; n = 8 comparison 68.0 ± 2.9 kg, body fat 25.0 ± 5.7%) NR Eight-week whole body anaerobic, agility and RT program,
four sessions/week
0.36 g/kg whey protein pre- and post-exercise (energy 83% protein, 14% CHO, 3% fat)
(daily protein intake NR)
0.35 g/kg casein protein pre- and post-exercise (energy 86% protein, 11% CHO, 4% fat) (daily protein intake NR) ↑ maximal strength, lean mass and anaerobic performance, and ↓ in fat mass in both groups

CK, creatine kinase; EAR, estimated average requirement; CHO, carbohydrate; whole body, includes upper and lower body; N/A, not applicable; NR, not reported; RDI, recommended dietary intake; RT, resistance training. 1 Differences refer to statistical significance reported in the study. 2 RDI calculated as 12% coefficient of variation (1.24 × EAR) in accordance with Rand et al. [35]. 3 Comparison group data did not meet the inclusion criteria.