Effect of combination treatment with high doses of MTF and GLB on the aerobic metabolism and ROI production in rod OS. Figure reports the effect of the combination treatment compared with the single treatment. The used treatments are: 5 mM MTF + 100 μM GLB and 5 mM MTF + 200 μM GLB. (A) Complex I activity, expressed as uU/mg of total protein. (B) ATP synthesis trough Fo–F1 ATP synthase expressed as nmol of ATP produced/min/mg of total protein. (C) ROI production in rod OS exposed to the light. Data are from n = 5 independent experiments. * or ** indicate a significant difference for p < 0.05 or 0.01, respectively, between the combination treatment and 5 mM MTF treatment. ### or #### indicate a significant difference for p < 0.001 or 0.0001, respectively, between the combination treatment and the respective exclusive GLB treatment.