Skip to main content
. 2020 Nov 10;25(22):5230. doi: 10.3390/molecules25225230

Figure 4.

Figure 4

Signal transduction of Rg3-RGE + PT by the NF-κB and MAPK pathways. RAW 264.7 cells were pre-incubated with Rg3-RGE + PT extract for 30 min and then stimulated with LPS for 18 h. Nuclear and cytosolic proteins from cells were extracted by NE-PER® nuclear and cytosolic extraction reagents. β-actin was used as an internal control. Images are representative of three independent experiments. (A,B) NF-κB pathway, and (C,D) MAPK pathway. Values in bar graphs represent mean ± SD of three independent experiments. *** p < 0.001, ** p < 0.05 and * p < 0.01 is considered significant compared to LPS and # p < 0.01 compared to inhibition by only Rg3-RGE or PT. Triplicates for Western blot analysis were completed within 3 weeks.