Figure 6.

Rg3-RGE + PT abolished the expression of pro-inflammatory mediators and cytokines in plasma of DSS mice. Plasma from blood was extracted and the expression of NO, TNF-α, IL-1β, IL-13 and IL-5 were determined by Enzyme Linked Immunosorbent Assay (ELISA) and colorimetric assay (A–E) where (A) NO expression, (B) TNF-α expression, (C) IL-1β expression, (D) IL-13 expression, and (E) IL-5 expression. Radar chart for normalized data trend fitting analysis of the serum biomarkers (F). Values in bar graphs represent mean ± SD of three independent experiments. *** p < 0.001 and ** p < 0.05 were considered significant compared to DSS group and ^# p < 0.01 compared to inhibition by only Rg3-RGE or PT. ELISA were conducted within 48 h of termination.