Figure 1.

Mesenchymal stromal cells (MSCs) decreased leukemic cell proliferation. Experiments were performed with KG1a leukemic cells cultured alone (blue), with MSC-CM (green), or in coculture with MSCs (red). (A) Experimental design of mono- and coculture of MSCs and leukemic KG1a cells. Leukemic cells were cultured in medium alone, with MSC-CM or over MSCs; MSCs were cultured alone or with KG1a leukemic cells. (B) Leukemic cell growth was evaluated after 72 h of mono-, MSC-CM, or coculture (nonadherent cells represent cells that do not adhere to MSCs after 72 h of coculture, n = 15). (C) Cell cycle was analyzed by a flow cytometry multilabeling protocol using anti-Ki67-AF488, anti-phosphoS10-H3-AF488 and 7AAD (n = 5). Variations in MSC-CM and cocultured vs. monocultured KG1a in cell cycle phases after 72h are shown on the left and a representative experiment is presented on the right. * p < 0.05.