Table 2.
FRAP units, trolox equivalent, and gallic acid equivalent obtained for a selected group of terpenes via FRAP assay modified by the addition of Tween 20.
| Terpenes | FRAP Units (Fe2+ µg·mL−1) | Trolox Equivalent (µg·mL−1) | Gallic Acid Equivalent (µg·mL−1) | |||
|---|---|---|---|---|---|---|
| 0.1 M | 1 mg·mL−1 | 0.1 M | 1 mg·mL−1 | 0.1 M | 1 mg·mL−1 | |
| γ-terpinene * | - | 1.38 (for 0.25 mg·mL−1) | - | 7.51 | - | 1.04 |
| citral | 3.21 | 1.04 | 7.43 | 2.52 | 1.03 | 0.32 |
| Carvone ** | False-positive results | 0.28 | False-positive results | 0.83 | False-positive results | 0.08 |
| α-phellandrene | 2.47 | 1.45 | 5.74 | 3.45 | 0.79 | 0.45 |
| α-terpinene *** | - | 5.75 (for 0.1 mg·mL−1) | - | 13.09 | 1.84 | |
| isopulegol | 0.38 | 0.01 | 1.06 | 0.05 | 0.11 | 0.01 |
| linalool | 0.34 | 0.09 | 0.96 | 0.40 | 0.09 | 0.01 |
| α-pinene | False-positive results **** | |||||
| farnesene | clouding/lack of color | |||||
| eucalyptol | clouding/lack of color | |||||
| terpinene-4-ol | clouding/lack of color | |||||
| p-cymene | clouding/lack of color | |||||
| β-myrcene | clouding/lack of color | |||||
| citronellal | clouding/lack of color | |||||
| menthol | clouding/lack of color | |||||
* γ-Terpinene: This terpene revealed high activity at a concentration of 0.1 M and 1 mg·mL−1, for which absorbance was >3.000. The measurements were performed at a concentration of 0.25 mg·mL−1. ** Carvone: Measurements were performed at a concentration of 0.5 mg·mL−1. *** α-Terpinene: This terpene exhibited high activity at a concentration of 0.1 M and 1 mg·mL−1, for which absorbance was >3.000. The measurements were performed at a concentration of 0.1 mg·mL−1. **** False-positive results: This effect was observed for probes with the blue color but clouding appeared simultaneously.