Figure 1.

GRA9 binds with NLRP3. (A) Identification of NLRP3 by mass spectrometry analysis in THP-1 cell lysates expressed with GRA9 or vector. (B) Vector or GRA9 expressing THP-1 cells were primed with LPS (100 ng·mL⁻¹) and stimulated with ATP or Nigericin (5 mM or 15 μM) for the indicated times, followed by immunoprecipitation (IP) with αFlag-agarose bead and immunoblotting (IB) with αNLRP3, αASC, αFlag, and αActin. The data are representative of four independent experiments with similar results (A,B). Full-length images of the blots presented in the Figure S1.