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. 2020 Nov 17;21(22):8644. doi: 10.3390/ijms21228644

Figure 6.

Figure 6

Bisphenol A-induced apoptosis in testicular germ cells. Terminal dUTP nick-end labeling (TUNEL) assay was performed to detect apoptotic germ cells in seminiferous epithelium (SE). (A) Images represent the position and number of TUNEL-positive germ cells (green) among other cells (blue; DAPI-stained) of SE from control, lowest-observed-adverse-effect level (LOAEL)-exposed, and ethinylestradiol (EE) groups. Scale bars = 50 µm. Bar graphs represent the percentages of apoptotic tubules in (B) F1, (C) F2, and (D) F3 offspring at their PND 120. Similarly, graphical presentation of TUNEL-positive germ cells per tubule in (E) F1, (F) F2, and (G) F3 offspring are also shown. Data were generated from at least 15 mice/group and analyzed using one-way analysis of variance (ANOVA) where * p < 0.05 and ** p < 0.01, compared to control.