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. 2020 Nov 10;9(11):1529. doi: 10.3390/plants9111529

Figure 4.

Figure 4

Molecular confirmation of transgenic lines overexpressing Os6PGDH1. (A) Southern blot of Os6PGDH1-overexpressing lines and WT plants. Genomic DNA from oe-15, oe-39, and WT plants was digested with EcoR I and Xba I, respectively. Digested genomic DNA was hybridized to a specific GUS probe. (B) Basal expression levels (means + SE, n = 5) of Os6PGDH1 in oe6PGDH lines and WT plants; (C,D) relative transcript levels (means + SE, n = 5) of Os6PGDH1 in oe6PGDH lines and WT plants that were mechanically wounded or infested by gravid BPH females for 12 h (these experiments were performed separately). Asterisks indicate significant differences in oe6PGDH lines compared with WT plants (one-way ANOVA followed by Duncan’s multiple range test, p < 0.05).