Table 1.
Co-differentially expressed genes in E. coli ATCC25922 induced by 1/2× MIC and 1/10× MIC of OLA.
| Gene Name | Function | Foldchange of Gene Expression | |
|---|---|---|---|
| (CvB) | (DvB) | ||
| Up-Regulated Genes | |||
| 1 Transcription Factors | |||
| malT | LuxR family transcriptional regulator, maltose regulon positive regulatory protein | 6.29 | 8.50 |
| soxS | AraC family transcriptional regulator, mar-sox-rob regulon activator | 4.16 | 4.65 |
| mlc | transcriptional regulator of PTS gene | 4.02 | 3.15 |
| 2 Oxidoreductases | |||
| wrbA | NAD(P)H dehydrogenase (quinone) | 2.60 | 5.15 |
| ahpC | alkyl hydroperoxide reductase | 2.12 | 4.85 |
| cydB | cytochrome bd ubiquinol oxidase subunit II | 2.14 | 4.37 |
| DR76_76 | oxidation-reduction process; coenzyme binding; oxidoreductase activity; mannitol metabolic | 2.60 | 3.68 |
| adhE | acetaldehyde dehydrogenase/alcohol dehydrogenase | 2.02 | 3.32 |
| icdA | isocitrate dehydrogenase | 2.80 | 3.09 |
| nuoM | NADH-quinone oxidoreductase subunit M | 2.30 | 3.07 |
| nuoI | NADH-quinone oxidoreductase subunit N | 2.34 | 2.95 |
| pfo | pyruvate-ferredoxin/flavodoxin oxidoreductase | 3.44 | 2.86 |
| grxB | glutaredoxin 2 | 2.06 | 2.83 |
| fdoI | formate dehydrogenase subunit gamma | 2.24 | 2.29 |
| fdnG | formate dehydrogenase major subunit | 2.27 | 2.23 |
| gcvP | glycine dehydrogenase | 2.28 | 2.16 |
| fdoH | formate dehydrogenase iron-sulfur subunit | 2.14 | 2.11 |
| 3 PTS system | |||
| manX | PTS system, mannose-specific IIAB component | 9.39 | 15.64 |
| manZ | PTS system, mannose-specific IID component | 8.80 | 12.58 |
| ptsG | glucose-specific PTS enzyme IIBC component | 8.73 | 12.52 |
| manY | PTS system, mannose-specific IIC component | 8.60 | 10.25 |
| ptsI | phosphotransferase system, enzyme I, PtsI | 2.78 | 2.68 |
| ptsH | phosphocarrier protein HPr | 2.70 | 2.32 |
| 4 Sugar and amino acid metabolism | |||
| asnA | aspartate-ammonia ligase | 2.39 | 6.20 |
| asnB | asparagine synthase (glutamine-hydrolysing) | 2.27 | 4.30 |
| gcvT | aminomethyl transferase | 2.16 | 3.39 |
| aspC | aspartate aminotransferase | 2.01 | 3.27 |
| purA | adenylosuccinate synthase | 2.09 | 2.61 |
| fbaA | fructose-bisphosphate aldolase, class II | 2.49 | 2.31 |
| 5 Others | |||
| bssR | biofilm regulator BssR | 2.46 | 5.36 |
| feoB | ferrous iron transport protein B | 2.38 | 4.14 |
| iraD | anti-adapter protein iraD | 4.62 | 3.24 |
| pflD | formate C-acetyltransferase | 3.63 | 2.89 |
| zraS | two-component system, NtrC family, sensor histidine kinase HydH | 2.60 | 2.05 |
| DR76_3474 | uncharacterized protein | 2.37 | 2.02 |
| Down-Regulated Genes | |||
| 1 DNA Binding and Repair | |||
| pgaA | DNA binding; negative regulation of DNA-templated transcription, termination | 0 | 0 |
| rusA | crossover junction endodeoxy ribonuclease RusA | 0 | 0.0066 |
| hyaE | DNA binding; DNA recombination | 0 | 0 |
| putP | DNA replication initiation | 0.0015 | 0.0033 |
| hybD | DNA recombination; DNA binding; DNA integration | 0.0020 | 0.0037 |
| bet | DNA binding; DNA metabolic process | 0.0031 | 0.0017 |
| putA | DNA replication | 0.0036 | 0.00071 |
| exo | DNA binding; nucleic acid phosphodiester bond hydrolysis; exonuclease activity | 0.0038 | 0.0024 |
| umuC | DNA polymerase V | 0.35 | 0.39 |
| 2 Membrane component | |||
| ompD | outer membrane porin protein LC | 0.0013 | 0.0012 |
| DR76_3645 | integral component of membrane | 0.0026 | 0 |
| DR76_3655 | integral component of membrane | 0.0028 | 0.010 |
| cea | cytolysis, integral component of membrane | 0.28 | 0.47 |
| 3 Others | |||
| DR76_3678 | hypothetical protein | 0 | 0.0051 |
| rutG | pyrimidine utilization transport protein G | 0.0047 | 0.0043 |
| yccM | putative electron transport protein | 0.014 | 0 |
| pepN | aminopeptidase N | 0.15 | 0.23 |
| higA | toxin HigB-1 | 0.16 | 0.12 |
| motA | chemotaxis protein MotA | 0.39 | 0.48 |
| tsr | methyl-accepting chemotaxis protein I, serine sensor receptor | 0.48 | 0.48 |
| pspA | phage shock protein A | 0.48 | 0.23 |
| pspG | phage shock protein G | 0.36 | 0.18 |
Note: CvB means 1/2× MIC OLA treated group compared with untreated group; DvB means 1/10× MIC OLA treated group compared with untreated group.