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. 2020 Nov 13;21(22):8561. doi: 10.3390/ijms21228561

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The SP receptor antagonist rescues the cell migration in response to TGF-β. (A,B) RP 67580 (RP; SP receptor antagonist; green dots) rescued the migration capacity of SP-pretreated ST2 cells in response to TGF-β stimulation. (C,D) CP-96345 (CP; SP receptor antagonist; green dots) rescued the migration capacity of SP-pretreated BM-MSCs in response to TGF-β stimulation. White arrows indicate the migrated cells on the lower membrane surface. Cells were stained with DAPI (blue) and the number of migrated cells is shown as a percentage of the total. The red lines indicate the mean value (p values were obtained by t-tests) and the scale bar represents 100 µm.