Table 1.
Primer sequences used for qPCR.
| Genes | Sequence 5′ to 3′ | Product Size (bp) | |
|---|---|---|---|
| T1α | F | TGC GAA AAA TGT CGG GAA GG | 51 |
| R | GGC GTA ACC CTT CAG CTC TT | ||
| SFTPB | F | GCC ATA CCA CAG GCA ATG CT | 80 |
| R | TGC TGC TCC ACA AAT TGC TT | ||
| SFTPC | F | CCT TCT TAT CGT GGT GGT GGT | 96 |
| R | TCT CCG TGT GTT TCT GGC TCA T | ||
| HOPX | F | GCC TTT CCG AGG AGG AGA C | 97 |
| R | TCT GTG ACG GAT CTG CAC TC | ||
| AQP5 | F | ACT GGG TTT TCT GGG TAG GG | 172 |
| R | ATG GTC TTC TTC CGC TCT TC | ||
| NKX2.1 | F | AGC ACA CGA CTC CGT TCT CA | 75 |
| R | CCT CCA TGC CCA CTT TCT TG | ||
| VIMENTIN | F | CCA GGC AAA GCA GGA GTC | 212 |
| R | CGA AGG TGA CGA GCC ATT | ||
| α-SMA | F | GAC GAA GCA CAG AGC AAA AG | 70 |
| R | AGT TGG TGA TGA TGC CAT GT | ||
| COL1A1 | F | AAG GGT GAG ACA GGC GAA CA | 70 |
| R | GAC CCT GGA GGC CAG AGA AG | ||
| CTNNB1 | F | AAA ATG GCA GTG CGT TTA | 99 |
| R | TTT GAA GGC AGT CTG TCG TA | ||
| TWIST1 | F | AGC AAG ATT CAG ACC CTC AAG | 145 |
| R | ATC CTC CAG ACC GAG AAG G | ||
| SNAIL1 | F | TTT ACC TTC CAG CAG CCC TA | 73 |
| R | GAC AGA GTC CCA GAT GAG CA | ||
| SNAIL2 | F | CTG TGG GGA CAT GAA CTG TG | 115 |
| R | AGG GTC TGG GGA AAC TCG | ||
| IL-1α | F | CCA ACG GGA AGG TTC TGA AG | 70 |
| R | GCC TCC AGG TCA TCA TCA GT | ||
| IL-1β | F | CTG TCC TGC GTG TTG AAA GA | 179 |
| R | TTC TGC TTG AGA GGT GCT GA | ||
| IL-6 | F | AGC CCT GAG AAA GGA GAC AT | 85 |
| R | TGG AAG GTT CAG GTT GTT TT | ||
| TNF-α | F | AAC CTC CTC TCT GCC ATC AA | 185 |
| R | CCA AAG TAG ACC TGC CCA GA | ||
| NOX1 | F | AGG GCT TTC GAA CAA CAA TA | 104 |
| R | CCA GCA CAG CCA CTT CAT AC | ||
| NOX2 | F | AAC TGC TGG AGA GCC AGA TG | 101 |
| R | GCA AAG TGA TTG GCC TGA GA | ||
| NOX3 | F | GCT ATG CAG AAT GGC AGA CA | 101 |
| R | TAC AAG ACC ACA GGG CCT AA | ||
| NOX4 | F | CTT TTG GAA GTC CAT TTG AG | 231 |
| R | GTC TGT TCT CTT GCC AAA AC | ||
| OCT4 | F | TCG AGA ACC GAG TGA GAG G | 125 |
| R | GAA CCA CAC TCG GAC CAC A | ||
| SOX2 | F | GCA CAT GAA GGA GCA CCC GGA TTA | 86 |
| R | GTG GTC CTT CTT GTG CTG C | ||
| NANOG | F | CAA AGG CAA ACA ACC CAC TT | 158 |
| R | TCT GCT GGA GGC TGA GGT AT | ||
| GADD45g | F | CAG ATC CAT TTT ACG CTG ATC CA | 209 |
| R | TCC TCG CAA AAC AGG CTG AG | ||
| SLUG | F | TGT GAC AAG GAA TAT GTG AGC C | 203 |
| R | TGA GCC CTC AGA TTT GAC CTG | ||
| MMP2 | F | AGC GAG TGG ATG CCG CCT TTA A | 138 |
| R | CAT TCC AGG CAT CTG CGA TGA G | ||
| MMP9 | F | CTC GAA CTT TGA CAG CGA CA | 187 |
| R | GCC ATT CAC GTC GTC CTT AT | ||
| ACE2 | F | GGGATCAGAGATCGGAAGAAGAAA | 124 |
| R | AGGAGGTCTGAACATCATCAGTG | ||
| TMPRSS2 | F | AATCGGTGTGTTCGCCTCTAC | 106 |
| R | CGTAGTTCTCGTTCCAGTCGT | ||
| GAPDH | F | GGC ATG GAC TGT GGT CAT GA | 87 |
| R | TGC ACC ACC AAC TGC TTA GC |