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. 2020 Nov 13;21(22):8560. doi: 10.3390/ijms21228560

Figure 6.

Figure 6

Adoptive transfer of Tregs from wild-type mice ameliorates imiquimod-induced psoriasiform dermatitis in TLR2-deficient mice. Shaved back skin and ears of TLR2 knockout (TLR2 KO) mice that had received Tregs from wild-type (WT) or TLR2 KO mice were topically treated with imiquimod for 5 consecutive days. (A) Phenotypical manifestation induced by imiquimod inTLR2 KO mouse that had received Tregs from WT mice, TLR2 KO mice, or PBS alone. (B) Disease severity and ear thickness during imiquimod treatment. Clinical scores for disease severity were calculated daily using a scoring system based on the clinical Psoriasis Area and Severity Index. Data are presented as mean ± SEM (n = 6 for each group). * p < 0.05 versus PBS treatment group. (C) Skin samples were taken 48h after imiquimod application. Messenger RNA levels of IL-17A, IFNγ, Foxp3, and IL-10 were determined by quantitative RT-PCR. Data were obtained from duplicate samples from 6 mice in each group. Values are presented as mean ± SEM of three independent experiments. * p < 0.05.