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. 2020 Nov 16;13(22):5155. doi: 10.3390/ma13225155

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Histological findings in the orthotopic experiments. (a) Histological finding of the TGF-β group in low-power magnification. TGC showed massive hard tissue formation as if it was added to the wall of the honeycomb TCP. (b) Histological findings of the TGF-β group in high-power magnification. Most of the TGC were observed to be irregularly arranged. (c) TGC showed a tendency to differentiate into odontoblasts because they were DSP positive. (d) Histological finding of the osteogenic medium group in low-power magnification. TGC showed massive hard tissue formation as if it was added to the wall of the honeycomb TCP. (e) Histological finding of the osteogenic medium group in high-power magnification. TGC which differentiated into highly columnar cells formed a single layer hard tissue so as to be added to the honeycomb TCP wall, and showed polarity with the nuclei arranged on the distal side of TCP. (f) TGC tended to differentiate into odontoblasts because they were DSP positive.