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. 2020 Nov 14;11(11):1351. doi: 10.3390/genes11111351

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Detection of the Vp7 antigen in the recombinant B. subtilis spores. (A) Western blot analysis of the Vp7 antigen in the spore coat proteins. Lane 1, the purified His₆-Vp28; lanes 2–6, the coat proteins extracted from the spores of the recombinant strains DR700, DR1947, DR1956/1985, DR2000/1985, and DR2000/2020, respectively. Molecular weight markers (kDa) are indicated at the right. (B) Dot blot analysis for the amount of the Vp7 antigen in the recombinant spore coat. The amounts of His₆-Vp7 (lane 1) and the coat proteins from the spores of DR700 (lane 2), DR1947 (lane 3), DR1956/1985 (lane 4), DR2000/1985 (lane 5), and DR2000/2020 (lane 6) were indicated. (C) Immunofluorescent detection of the Vp7 antigen on the spore surface. The immunofluorescence-labeled spores were visualized under bright field (b) and fluorescence (f) microscopy. Scale bars indicate 1 μm. (D) Quantitative analysis of the Cy5 fluorescence on the spore surface with fluorospectrophotometer.