Table 1.
Plasmids and strains used in this paper.
| Plasmid | Characteristic Property or Genotype | Origen or Reference | |
|---|---|---|---|
| pJS700 | An integrative vector containing the integrative fragment amyE::erm-cotB | [25] | |
| pJS1947 | A derivative of pJS700, containing the integrative fragment amyE::erm-cotB-vp7 | This study | |
| pJS1956 | An integrative vector containing the integrative fragment cwlJ::erm-cotB | This study | |
| pJS1985 | An integrative vector containing the integrative fragment sleB::npt-cotC | This study | |
| pJS2000 | A derivative of pJS2016, containing the integrative fragment cwlJ::erm-cotB-vp7 | This study | |
| pJS2020 | A derivative of pJS1976, containing the integrative fragment sleB::npt-cotC-vp7 | This study | |
| pJS1621 | The vp7 gene in pET28a | This study | |
| B. subtilis | Relevant Genotype | Expressed Fusion Antigen 1 on Spore Surface | Origen or Reference |
| 168 (trp-) | Wild type (WT) | - | BGSC 2 |
| DR700 | amyE::erm-cotB | - | This study |
| DR1947 | amyE:erm-cotB-vp7 | CotB-Vp7, 60.7 kDa | This study |
| DR1956/1985 | cwlJ::erm-cotB; SleB::npt-cotC | - | This study |
| DR2000/1985 | cwlJ::erm-cotB-vp7; sleB::npt-cotC | CotB-Vp7, 60.7 kDa | This study |
| DR2000/2020 | cwlJ::erm-cotB-vp7; sleB::npt-cotC-vp7 | CotB-Vp7, 60.7 kDa; CotC-Vp7, 44.4 kDa | This study |
1 Molecular weight of protein chimera was estimated according to its amino acid sequence and determined by SDS-PAGE analysis. 2 Bacillus Genetic Stock Center.