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. 2020 Nov 16;21(22):8628. doi: 10.3390/ijms21228628

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of alpha-1 antitrypsin on α-SMA staining and expression in tacrolimus-induced renal injury. (A) Immunohistochemical staining for α-SMA (200× magnification) (Left). Quantitative analysis of α-SMA staining area (Right). (B) Immunoblotting analysis of α-SMA. Data are presented as means ± standard errors; ** p < 0.01 vs. VH; *** p < 0.001 vs. VH; ^## p < 0.01 vs. TAC; ^### p < 0.001 vs. TAC. Abbreviations: VH, vehicle group; AAT, alpha-1 antitrypsin treatment group; TAC, tacrolimus treatment group; TAC + AAT, tacrolimus and alpha-1 antitrypsin cotreatment group.