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. 2020 Nov 13;11(11):1343. doi: 10.3390/genes11111343

Figure 1.

Figure 1

Reinforced proliferation and impairment of migration ability of FlncKO line. (A) Proliferation dynamics was obtained by xCELLigence Real-Time Cell Analyzer (RTCA), normalized cell index (CI) curves are presented. (B) Quantification of doubling time in 15 to 43 hours’ time range revealed an increase of proliferation rate in FlncKO cell line (n = 15 for each cell line). (C) Scratch assay shows a decrease of migration capacity from FlncKO cell line compared to scramble control; cell migration was recorded from 2 to 12 h. Representative images are shown for 12 hours’ time point. Dashed lines indicate area for quantification. (D) Reduction in cell migration was significant from 6 hours’ time point until 12 hours’ time point (n = 4 for each cell line and time points). (E) Validation of migration results performed by RTCA analysis. Migration curves represent cell index in each time point. (F). Quantification analysis of migration curves slopes was performed between 15 to 23 h after cell seeding. Scale bar 200 μM. For all * p < 0.05.