Figure 1.

Experimental design. After acclimatization for 1 week, 5-week-old male C57BL/6J mice were fed with a high-fat diet (HFD) or standard chow diet for 4 weeks. At the 5th week, male C57BL/6J mice were injected with streptozotocin (STZ) intraperitoneally at the dose of 90 mg/kg body weight (BW). One week after STZ injection, fasting blood glucose (FBG) was measured and mice with FBG levels > 11.1 mmol/L were considered as diabetic mice, and randomly allocated to the model control group (MC, administrated with saline by oral gavage) or the prolamin from cooked foxtail millet group (PCFM, administrated with 200 mg/kg BW PCFM by oral gavage). After five weeks’ treatment, the effects of PCFM on the diabetes-related biomarkers, gut microbiota and serum metabolic profiles of diabetic mice were evaluated.