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. 2020 Nov 11;9(11):1109. doi: 10.3390/antiox9111109

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The increased glycolysis and metabolic enzyme activities of activated macrophages are reduced by hemin. (A) Glycolysis and the activities of (B) hexokinase, (C) pyruvate kinase, and (D) glucose-6-phosphate dehydrogenase were measured in macrophages and activated macrophages, in the absence or presence of 10 µM hemin for 72 h. Activities of the enzymes were expressed as unit/mg protein, calculated as nmol of NADP reduced/min/mg protein for hexokinase and glucose-6-phosphate dehydrogenase, and as nmol of NADH oxidized/min/mg protein for pyruvate kinase. Values with different superscript letters (a and b) indicate significant differences (p < 0.05) between groups. For all bars with the same letter, the difference between the means is not statistically significant. G6PDH, glucose-6-phosphate dehydrogenase.

The increased glycolysis and metabolic enzyme activities of activated macrophages are reduced by hemin. (A) Glycolysis and the activities of (B) hexokinase, (C) pyruvate kinase, and (D) glucose-6-phosphate dehydrogenase were measured in macrophages and activated macrophages, in the absence or presence of 10 µM hemin for 72 h. Activities of the enzymes were expressed as unit/mg protein, calculated as nmol of NADP reduced/min/mg protein for hexokinase and glucose-6-phosphate dehydrogenase, and as nmol of NADH oxidized/min/mg protein for pyruvate kinase. Values with different superscript letters (a and b) indicate significant differences (p < 0.05) between groups. For all bars with the same letter, the difference between the means is not statistically significant. G6PDH, glucose-6-phosphate dehydrogenase.