Table 1.
Stretch of residues assigned to the defined regions of each predicted hairpin motif
| Predicted hairpins | N-terminus | H1 top | H1 mid | Hinge | H2 mid | H2 top | C-terminus |
|---|---|---|---|---|---|---|---|
| LiveDrop | HYE | FISWKITSI | WVFGFFIRY | VILMPLRVL | VCFVGVVWL | TVCTAAVG | YLKDGPFKR |
| ALG14 hp | QS | WLSSIFTSL | WALLWSCYLV | WRDRPQLILCN | GPGTCVPFCYAA | YLWRLLGRL | PS |
| Spastin hp | GYSSSVHKQN | LYVVSF | PIIFLF | NVLRSLI | YQLFCI | FRYLYG | ASTKVIYRPH |
| LDAH hp | MMESPNG | WVFTKVA | MPLYSVFGYIF | FSFFNFLPVWLR | LMLIQIYFLIF | SIPRQFL | GTALKYS |
| AGPAT3 hp | RLCS | LVNFVCW | AVFSLSCIFY | YVITSLLAANW | TAFITALSVL | GLFYWLM | GQAINK |
| DGAT2 hp | RLQ | ILVTAFF | TSMLLILLSVS | FLLVAGSLIYGG | LLVRSLMVTY | LAYVFVH | HKKTQS |
| FATP hp | QRRRRQR | FLVIFRFFC | ATVAFGLAIACV | IYTLHTMGW | IFAVLVALVALL | LTKPGWRWF | YIAGATA |
| SelT hp | DPPGLNYYLS | KMIFALKII | IIVSVVSAVSPF | TFLGLNTPSWW | SHMQANKIYACM | MIFFLGNML | EAQLISSGA |
The amino acid sequence of each of the predicted hairpin motifs was divided into five regions. In each case, the predicted α-helices (H1 and H2) were divided into top (H1 top, H2 top) and middle (H1 mid, H2 mid) regions. The residues predicted to connect the α-helices were defined as the hinge region. Short sequence overhangs at the N- and C-termini were included when expressing these motifs in cells. The tryptophan and positively charged residues are underlined.