KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-mCherry | Takara | Cat# 632543; RRID: AB_2307319 |
| Mouse monoclonal anti-ubiquitin | Cell Signaling Technology | Cat# 3936S; RRID: AB_331292 |
| Mouse monoclonal anti-α-tubulin | Sigma-Aldrich | Cat# T5168; RRID: AB_477579 |
| Mouse monoclonal anti-FLAG | Sigma-Aldrich | Cat# F1804; RRID: AB_262044 |
| Polyclonal rabbit anti-GRP78/BiP | Sigma-Aldrich | Cat# G9043; RRID: AB_2279879 |
| Mouse anti-IgG kappa binding protein-HRP | Santa Cruz Biotechnology | Cat# sc-516102; RRID: AB_2687626 |
| Mouse monoclonal anti-rabbit IgG-HRP | Santa Cruz Biotechnology | Cat# sc-2357; RRID: AB_628497 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Effectene® Transfection Reagent | Qiagen | Cat# 301425 |
| Oleic acid | Sigma-Aldrich | Cat# O1383 |
| Concanavalin A | Sigma-Aldrich | Cat# C5275 |
| BODIPY™ 493/503 | Thermo Fisher Scientific | Cat# D3922 |
| CB-5083, VCP/p97 inhibitor | Selleck Chemicals | Cat# S8101 |
| Triton™ X-100 | Sigma-Aldrich | Cat# T8787 |
| cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail | Roche | Cat# 11836170001 |
| Trypsin from bovine pancreas | Sigma-Aldrich | Cat# T9201 |
| Trypsin inhibitor from Glycine max (soybean) | Sigma-Aldrich | Cat# T9003 |
| Critical Commercial Assays | ||
| pENTR™/SD/D-TOPO™ Cloning Kit | Thermo Fisher Scientific | Cat# K242020 |
| Gateway™ LR Clonase™ II Enzyme mix | Thermo Fisher Scientific | Cat# 11791020 |
| QuikChange II XL Site-Directed Mutagenesis Kit | Agilent Technologies | Cat# 200521 |
| Pierce™ BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23225 |
| SuperSignal™ West Pico | Thermo Fisher Scientific | Cat# 34580 |
| SuperSignal™ West Dura | Thermo Fisher Scientific | Cat# 34076 |
| SuperSignal™ West Femto | Thermo Fisher Scientific | Cat# 34095 |
| Experimental Models: Cell Lines | ||
| D. melanogaster: Cell line S2: S2R+ | Drosophila RNAi Screening Center, Laboratory of Norbert Perrimon | FlyBase ID: FBtc0000150; RRID: CVCL_Z831 |
| Oligonucleotides | ||
| Primers for site-directed mutagenesis, see Table S4 | This paper | N/A |
| Recombinant DNA | ||
| Plasmid: pA-CherryW | Guo et al., 2008 | N/A |
| Synthetic double-stranded DNA: gBlocks® Gene Fragments | Integrated DNA Technologies | N/A |
| Software and Algorithms | ||
| TOPCONS | Tsirigos et al., 2015 | http://topcons.cbr.su.se |
| PSIPRED | Jones, 1999 | http://bioinf.cs.ucl.ac.uk/psipred/ |
| NIS-Elements | Nikon | N/A |
| Clustal Omega | Sievers et al., 2011 | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| Jalview | Waterhouse et al., 2009 | http://www.jalview.org/ |
| EVcouplings | Marks et al., 2011 | https://evcouplings.org/ |
| HELIQUEST | Gautier et al., 2008 | http://heliquest.ipmc.cnrs.fr/ |
| Rosetta ab initio structure generation tool | Rohl et al., 2004 | https://rosettacommons.org/ |
| Robetta server | Kim et al., 2004 | https://robetta.bakerlab.org/ |
| RosettaMP | Alford et al., 2015 | https://rosettacommons.org/ |
| OCTOPUS | Viklund and Elofsson, 2008 | http://octopus.cbr.su.se/ |
| CHARMM-GUI | Jo et al., 2008 | http://www.charmm-gui.org/ |
| VMD | Humphrey et al., 1996 | https://www.ks.uiuc.edu/Research/vmd/ |
| Packmol | Martinez et al., 2009 | http://m3g.iqm.unicamp.br/packmol/home.shtml |
| GROMACS | Abraham et al., 2015 | http://manual.gromacs.org/ |
| CHARMM36m force field | Klauda et al., 2010 | http://mackerell.umaryland.edu/charmm_ff.shtml |
| Plumed2 | Tribello et al., 2014 | https://www.plumed.org/ |
| Weighted Histogram Analysis Method (WHAM) package | Laboratory of Alan Grossfield | http://membrane.urmc.rochester.edu/?page_id=126 |
| Fiji | Schindelin et al., 2012 | https://fiji.sc/ |
| GraphPad Prism 8 | GraphPad | http://www.graphpad.com/ |
| R (version 3.6.0) | R Core Team | https://www.r-project.org/ |