Figure 2. BA-DGAT-KO Mice Maintain Euthermia during Acute or Chronic Cold Exposure.

(A) Mice were exposed to cold acutely (in ad libitum fed or in fasted state) or chronically for 1 week (n = 8).

(B) Glycogen levels in brown fat (n = 3).

(C) mRNA levels in BAT of mice housed at room temperature (n = 6).

(D) Glycogen synthase activity in BAT measured in vitro (n = 5).

(E) Plasma levels of free fatty acids (n = 7).

(F) [¹⁴C]-Deoxyglucose uptake by tissues in vivo in basal or CL 316,243-administered mice (n = 3).

(G) [¹⁸F]-FDG-PET/CT scans of CL 316,243-administered mice.

(H) Western blot analysis of glucose transporters in whole tissue homogenate and in plasma membrane fractions isolated from BAT of insulin-administered mice (n = 6).

(I) Western blot analysis of tyrosine hydroxylase in BAT of room temperature-housed or cold-exposed mice (n = 3).

(J) Western blot analysis of insulin signaling in BAT of basal or CL 316,243-administered mice (n = 3).

Data are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.