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. Author manuscript; available in PMC: 2020 Nov 28.
Published in final edited form as: Cell Rep. 2020 Nov 3;33(5):108348. doi: 10.1016/j.celrep.2020.108348

Figure 3. Adaptations of BAT Lacking TG Stores to Maintain Thermogenesis.

Figure 3.

(A) mRNA levels in BAT of mice housed at room temperature (n = 6).

(B) mRNA levels in BAT of ad libitum fed or cold-exposed (fasted) mice (n = 6).

(C) Total protein content of isolated mitochondria (n = 8).

(D) Western blot analysis of mitochondrial proteins from BAT tissue lysates (n = 4).

(E) Western blot analysis of mitochondrial proteins from isolated mitochondria (n = 4).

(F and G) Oxygen consumption rates (OCR) of isolated mitochondria measured using glycerol-3-phosphate or pyruvate as substrates. The difference between initial respiration and respiration after GDP addition was considered UCP1 activity. The difference between respiration after ADP addition and after oligomycin addition was considered ATP synthase activity (n = 4).

(H) mRNA levels of Ucp1 in iWAT of room temperature-housed or acutely cold-exposed mice (n = 6).

(I) Western blot analysis of UCP1 and tyrosine hydroxylase (TH) in iWAT of room temperature-housed or cold-exposed mice (n = 3).

(J) Levels of OXPHOS proteins in iWAT of room temperature-housed or cold-exposed mice (n = 3).

Data are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.